Soybean Mosaic Virus and Purposeful Identification of a Key Amino Acid

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jdomenech

In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline

The Escherichia coli and vaccinia virus-based reverse genetics strategies have been broadly utilized for the manipulation and engineering of coronavirus genomes. These strategies, nonetheless, present numerous limitations and are usually robust to find out in a nicely timed technique for (re-)rising viruses. On this chapter, we present a model new frequent reverse genetics platform for the assembly and engineering of infectious full-length cDNAs using yeast-based transformation-associated recombination cloning.

This novel assembly approach not solely ends in safe coronavirus infectious full-length cDNAs cloned inside the yeast Saccharomyces cerevisiae however moreover fosters and accelerates the manipulation of their genomes.

Such a platform is broadly related for the scientific neighborhood, as a result of it requires no specific gear and might be carried out in an odd laboratory setting. The protocol described might be merely tailor-made to simply about all acknowledged or rising coronaviruses, paying homage to Heart East respiratory syndrome coronavirus (MERS-CoV). 

jdomenech
jdomenech

cDNA from Monkey (Rhesus) Normal Tissue: Brain
C1534035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Cecum
C1534089 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Colon
C1534090 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Epididymis
C1534105 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Esophagus
C1534106 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Gallbladder
C1534118 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Heart
C1534122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Kidney
C1534142 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Liver
C1534149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Lung
C1534152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Trachea
C1534160 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Diaphragm
C1534169 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Ovary
C1534183 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Pancreas
C1534188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Parathyroid
C1534189 40 reactions
EUR 1051
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Parotid
C1534190 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Penis
C1534194 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Placenta
C1534200 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Prostate
C1534201 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Rectum
C1534206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Skin
C1534218 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Spleen
C1534246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Stomach
C1534248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Testis
C1534260 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Throat
C1534263 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Thymus
C1534264 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Thyroid
C1534265 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Tongue
C1534267 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Tonsil
C1534268 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Ureter
C1534273 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Uterus
C1534274 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Vagina
C1534283 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Colon Ascending
C1534091 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Colon descending
C1534092 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Colon Sigmoid
C1534095 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Colon Transverse
C1534096 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Ductus Deferens
C1534100 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Fallopian Tube
C1534115 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Heart: Pericardium
C1534133 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Lymph Node
C1534161 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Skeletal Muscle
C1534171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine
C1534226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Spinal Cord
C1534234 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Stomach: Cardia
C1534250 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Stomach: Corpus
C1534251 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Stomach: Fundus
C1534252 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Stomach: Pylorus
C1534253 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Cervix
C1534275 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Corpus
C1534276 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Fundus
C1534278 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Blood Vessel: Artery
C1534013 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Blood Vessel: Vein
C1534020 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum
C1534101 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Liver: Left Lobe
C1534150 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Liver: Right Lobe
C1534151 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum
C1534227 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum
C1534230 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
SAA1, monkey (rhesus macaque)
RC326-12 2ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Other
Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey)
4-PAA033Si01
  • EUR 270.00
  • EUR 2879.00
  • EUR 709.00
  • EUR 343.00
  • EUR 224.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa)
Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey)
4-PAA546Si01
  • EUR 310.00
  • EUR 3500.00
  • EUR 850.00
  • EUR 400.00
  • EUR 241.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA)
Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey)
4-PAA979Si01
  • EUR 270.00
  • EUR 2879.00
  • EUR 709.00
  • EUR 343.00
  • EUR 224.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CYPA (Val2~Glu165)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA)
Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey)
4-MAA080Si21
  • EUR 259.00
  • EUR 2708.00
  • EUR 670.00
  • EUR 328.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Ala23~Pro101
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8)
Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey)
4-MAA080Si22
  • EUR 259.00
  • EUR 2708.00
  • EUR 670.00
  • EUR 328.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8)
Interleukin 5 (IL5) Polyclonal Antibody (Rhesus monkey)
4-PAA078Si01
  • EUR 269.00
  • EUR 2866.00
  • EUR 706.00
  • EUR 342.00
  • EUR 223.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: IL5 (Ile20~Ser134)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5)
Interleukin 6 (IL6) Polyclonal Antibody (Rhesus monkey)
4-PAA079Si01
  • EUR 270.00
  • EUR 2879.00
  • EUR 709.00
  • EUR 343.00
  • EUR 224.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: IL6 (Pro27~Met212)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6)
Interleukin 8 (IL8) Polyclonal Antibody (Rhesus monkey)
4-PAA080Si01
  • EUR 251.00
  • EUR 2576.00
  • EUR 640.00
  • EUR 316.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: IL8 (Ala23~Pro101)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8)
Cathepsin K (CTSK) Monoclonal Antibody (Rhesus monkey)
4-MAA267Si21
  • EUR 278.00
  • EUR 3011.00
  • EUR 739.00
  • EUR 355.00
  • EUR 227.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Pro116~Met329
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK)
Monkey CD4 ELISA Kit
EMKC0011 96Tests
EUR 521
Monoclonal CD4 Antibody (clone 5D9), Clone: 5D9
AMM02279G 0.05ml
EUR 484
Description: A Monoclonal antibody against Human CD4 (clone 5D9). The antibodies are raised in Mouse and are from clone 5D9. This antibody is applicable in WB and IHC-P
Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), APC
4-PAA033Si01-APC
  • EUR 380.00
  • EUR 3779.00
  • EUR 1038.00
  • EUR 490.00
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with APC.
Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), Biotinylated
4-PAA033Si01-Biotin
  • EUR 337.00
  • EUR 2829.00
  • EUR 819.00
  • EUR 417.00
  • EUR 230.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with Biotin.
Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), Cy3
4-PAA033Si01-Cy3
  • EUR 465.00
  • EUR 4997.00
  • EUR 1343.00
  • EUR 612.00
  • EUR 271.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with Cy3.
Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), FITC
4-PAA033Si01-FITC
  • EUR 324.00
  • EUR 3043.00
  • EUR 850.00
  • EUR 412.00
  • EUR 207.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with FITC.
Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), HRP
4-PAA033Si01-HRP
  • EUR 346.00
  • EUR 3291.00
  • EUR 916.00
  • EUR 441.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with HRP.
Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), PE
4-PAA033Si01-PE
  • EUR 324.00
  • EUR 3043.00
  • EUR 850.00
  • EUR 412.00
  • EUR 207.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with PE.
Glycated Hemoglobin A1c (HbA1c) Polyclonal Antibody (Rhesus monkey)
4-PAA190Si01
  • EUR 269.00
  • EUR 2866.00
  • EUR 706.00
  • EUR 342.00
  • EUR 223.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Native Protein
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c)
Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), APC
4-PAA546Si01-APC
  • EUR 440.00
  • EUR 4625.00
  • EUR 1250.00
  • EUR 575.00
  • EUR 260.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with APC.
Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), Biotinylated
4-PAA546Si01-Biotin
  • EUR 381.00
  • EUR 3450.00
  • EUR 975.00
  • EUR 480.00
  • EUR 249.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with Biotin.
Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), Cy3
4-PAA546Si01-Cy3
  • EUR 545.00
  • EUR 6125.00
  • EUR 1625.00
  • EUR 725.00
  • EUR 305.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with Cy3.
Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), FITC
4-PAA546Si01-FITC
  • EUR 372.00
  • EUR 3720.00
  • EUR 1020.00
  • EUR 480.00
  • EUR 228.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with FITC.
Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), HRP
4-PAA546Si01-HRP
  • EUR 398.00
  • EUR 4025.00
  • EUR 1100.00
  • EUR 515.00
  • EUR 242.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with HRP.
Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), PE
4-PAA546Si01-PE
  • EUR 372.00
  • EUR 3720.00
  • EUR 1020.00
  • EUR 480.00
  • EUR 228.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with PE.
Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), APC
4-PAA979Si01-APC
  • EUR 380.00
  • EUR 3779.00
  • EUR 1038.00
  • EUR 490.00
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CYPA (Val2~Glu165)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with APC.
Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), Biotinylated
4-PAA979Si01-Biotin
  • EUR 337.00
  • EUR 2829.00
  • EUR 819.00
  • EUR 417.00
  • EUR 230.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CYPA (Val2~Glu165)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with Biotin.
Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), Cy3
4-PAA979Si01-Cy3
  • EUR 465.00
  • EUR 4997.00
  • EUR 1343.00
  • EUR 612.00
  • EUR 271.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CYPA (Val2~Glu165)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with Cy3.
Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), FITC
4-PAA979Si01-FITC
  • EUR 324.00
  • EUR 3043.00
  • EUR 850.00
  • EUR 412.00
  • EUR 207.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CYPA (Val2~Glu165)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with FITC.
Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), HRP
4-PAA979Si01-HRP
  • EUR 346.00
  • EUR 3291.00
  • EUR 916.00
  • EUR 441.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CYPA (Val2~Glu165)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with HRP.
Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), PE
4-PAA979Si01-PE
  • EUR 324.00
  • EUR 3043.00
  • EUR 850.00
  • EUR 412.00
  • EUR 207.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CYPA (Val2~Glu165)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with PE.
Hemoglobin (HB) Polyclonal Antibody (Human, Rhesus monkey, Dog)
4-PAB409Si01
  • EUR 280.00
  • EUR 3024.00
  • EUR 742.00
  • EUR 356.00
  • EUR 227.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Native Protein Hemoglobin
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB)
Carbonic Anhydrase II (CA2) Monoclonal Antibody (Rhesus monkey)
4-MAA782Si22
  • EUR 259.00
  • EUR 2708.00
  • EUR 670.00
  • EUR 328.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Inquire for antigen sequence.
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2)
Interleukin 6 (IL6) Monoclonal Antibody (Human, Rhesus monkey)
4-MAA079Si22
  • EUR 278.00
  • EUR 3011.00
  • EUR 739.00
  • EUR 355.00
  • EUR 227.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Pro27~Met212
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Mouse monoclonal antibody against Human, Rhesus monkey Interleukin 6 (IL6)
Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), APC
4-MAA080Si21-APC
  • EUR 364.00
  • EUR 3545.00
  • EUR 980.00
  • EUR 467.00
  • EUR 227.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Ala23~Pro101
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with APC.
Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), Biotinylated
4-MAA080Si21-Biotin
  • EUR 325.00
  • EUR 2658.00
  • EUR 777.00
  • EUR 400.00
  • EUR 225.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Ala23~Pro101
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Biotin.
Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), Cy3
4-MAA080Si21-Cy3
  • EUR 444.00
  • EUR 4685.00
  • EUR 1265.00
  • EUR 581.00
  • EUR 261.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Ala23~Pro101
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Cy3.
Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), FITC
4-MAA080Si21-FITC
  • EUR 311.00
  • EUR 2856.00
  • EUR 804.00
  • EUR 393.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: Ala23~Pro101
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with FITC.
 

Synthesis of Full-Measurement cDNA Infectious Clones of Soybean Mosaic Virus and Purposeful Identification of a Key Amino Acid inside the Silencing Suppressor Hc-Skilled 

  • Soybean mosaic virus (SMV), which belongs to the Potyviridae, causes important reductions in soybean yield and seed top quality. On this look at, every tag-free and reporter gene inexperienced fluorescent protein (GFP)-containing infectious clones for the SMV N1 strain have been constructed by Gibson assembly and with the yeast homologous recombination system, respectively.
  • Every infectious clones are acceptable for agroinfiltration on the model host benthamiana and current sturdy infectivity for the pure host soybean and several other different totally different legume species. Every infectious clones have been seed transmitted and prompted typical virus indicators on seeds and progeny crops. We used the SMV-GFP infectious clone to extra look at the perform of key amino acids inside the silencing suppressor helper component-proteinase (Hc-Skilled).
  • Amongst twelve amino acid substitution mutants, the co-expression of mutant 2-with an Asparagine→Leucine substitution at place 182 of the FRNK (Phe-Arg-Asn-Lys) motif-attenuated viral indicators and alleviated the host progress retardation introduced on by SMV.
  • Moreover, the Hc-Prom2 mutant confirmed stronger oligomerization than wild-type Hc-Skilled. Taken collectively, the SMV infectious clones may be useful for analysis of host-SMV interactions and helpful gene characterization in soybeans and related legume species, notably by means of seed transmission properties. Furthermore, the SMV-GFP infectious clone will even facilitate helpful analysis of every virus and host genes in an benthamiana transient expression system. 

Profiling of rice Cd-tolerant genes by means of yeast-based cDNA library survival screening

The bioaccumulation of cadmium (Cd) in crop and the following meals chain has aroused intensive concerns. However, the underlying molecular mechanisms of plant Cd tolerance keep to be clarified from the viewpoint of novel candidate genes.

 

Proper right here we described a extraordinarily setting pleasant technique for preliminary determining rice Cd-tolerant genes by means of the yeast-based cDNA library survival screening blended with high-throughput sequencing approach. About 690 gene isoforms have been acknowledged as being Cd-tolerant candidates using this shotgun technique.

Among the many many Cd-tolerant genes acknowledged, numerous courses of genes paying homage to BAX inhibitor (BI), NAC transcription parts and Quick ALkalinization Parts (RALFs) have been of particular curiosity, and their carry out of Cd tolerance was extra validated by way of heterologous expression, which urged that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves.

Referring to the genes involved in ion transport, the validated Cd-tolerant heavy metal-associated space (HMAD) isoprenylated protein HIPP42 was considerably noteworthy. Further elucidation of these genes associated to Cd tolerance in rice will revenue agricultural actions.

Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Period for scRNA-Seq

 

Single-cell RNA-sequencing (scRNA-seq) permits a whole analysis of the transcriptome of specific particular person cells by next-generation sequencing. ScRNA-seq offers an unbiased technique to research the cell heterogeneity and dynamics of varied natural strategies, along with the immune system. Optimization of the technical procedures carried out earlier to RNA-seq analysis is essential to the success of a scRNA-seq experiment.

Proper right here, three major experimental procedures are described: (1) the isolation of immune CD8a+ T cells from major murine tissue, (2) the expertise of single-cell cDNA libraries using the 10× Genomics Chromium Controller and the Chromium Single Cell 3′ Reply, and (3) cDNA library top quality administration. On this protocol, CD8a+ T cells are isolated from murine spleen tissue, nonetheless any cell form of curiosity might be enriched and used for single-cell cDNA library expertise and subsequent RNA-seq experiments.

Combinatorial dynamics of protein synthesis time delay and detrimental suggestions loop in NF- κ B signalling pathway

The transcription issue NF-κB hyperlinks immune response and inflammatory response and its totally different oscillation patterns decide totally different cell fates. On this research, a mathematical mannequin with IκBα protein synthesis time delay is developed primarily based on the experimental evidences.

The outcomes present that point delay has the flexibility to drive oscillation of NF-κB through Hopf bifurcation. In the meantime, the amplitude and interval are delicate to the time delay. Furthermore, the time delay threshold is a perform of 4 parameters characterising the detrimental suggestions loop.

Likewise, the parameters additionally affect the amplitude and interval of NF-κB oscillation induced by time delay. Subsequently, the oscillation patterns of NF-κB are collaborative outcomes of time delay coupled with the detrimental suggestions loop. These outcomes not solely improve the understanding of NF-κB organic oscillation but in addition present clues for the event of anti-inflammatory or anti-cancer medicine.

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