Soybean Mosaic Virus and Purposeful Identification of a Key Amino Acid

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jdomenech

In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline

The Escherichia coli and vaccinia virus-based reverse genetics strategies have been broadly utilized for the manipulation and engineering of coronavirus genomes. These strategies, nonetheless, present numerous limitations and are usually robust to find out in a nicely timed technique for (re-)rising viruses. On this chapter, we present a model new frequent reverse genetics platform for the assembly and engineering of infectious full-length cDNAs using yeast-based transformation-associated recombination cloning.

This novel assembly approach not solely ends in safe coronavirus infectious full-length cDNAs cloned inside the yeast Saccharomyces cerevisiae however moreover fosters and accelerates the manipulation of their genomes.

Such a platform is broadly related for the scientific neighborhood, as a result of it requires no specific gear and might be carried out in an odd laboratory setting. The protocol described might be merely tailor-made to simply about all acknowledged or rising coronaviruses, paying homage to Heart East respiratory syndrome coronavirus (MERS-CoV). 

jdomenech
jdomenech

Monkey Tongue cDNA, Rhesus

UD-105 30 reactions
EUR 316

Monkey Rectum cDNA, Rhesus

UD-312 30 reactions
EUR 316

Monkey Testis cDNA, Rhesus

UD-401 30 reactions
EUR 316

Monkey Spleen cDNA, Rhesus

UD-701 30 reactions
EUR 316

Monkey Thymus cDNA, Rhesus

UD-702 30 reactions
EUR 316

Monkey Tonsil cDNA, Rhesus

UD-707 30 reactions
EUR 446

Monkey Artery cDNA, Rhesus

UD-810 30 reactions
EUR 446

Monkey Kidney cDNA, Rhesus

UD-901 30 reactions
EUR 316

Monkey Stomach cDNA, Rhesus

UD-302 30 reactions
EUR 316

Monkey Adrenal cDNA, Rhesus

UD-501 30 reactions
EUR 446

Monkey Thyroid cDNA, Rhesus

UD-503 30 reactions
EUR 446

Monkey Trachea cDNA, Rhesus

UD-602 30 reactions
EUR 446

Monkey Bladder cDNA, Rhesus

UD-902 30 reactions
EUR 446

Monkey Prostate cDNA, Rhesus

UD-408 30 reactions
EUR 316

Monkey Esophagus cDNA, Rhesus

UD-301 30 reactions
EUR 316

Monkey Pituitary cDNA, Rhesus

UD-502 30 reactions
EUR 446

Monkey Diaphragm cDNA, Rhesus

UD-605 30 reactions
EUR 316

Monkey Epididymis cDNA, Rhesus

UD-402 30 reactions
EUR 316

Monkey Spinal cord cDNA, Rhesus

UD-230 30 reactions
EUR 316

Monkey Lymph Nodes cDNA, Rhesus

UD-703 30 reactions
EUR 446

Monkey Bone Marrow cDNA, Rhesus

UD-704 30 reactions
EUR 446

Monkey (Rhesus) cDNA Tissue: Thyroid

MR34-265 10 rxn
EUR 498

Monkey Adipose Tissue cDNA, Rhesus

UD-103 30 reactions
EUR 446

Monkey Salivary gland, cDNA, Rhesus

UD-317 30 reactions
EUR 316

Monkey Skeletal Muscles cDNA, Rhesus

UD-102 30 reactions
EUR 316

Monkey Seminal Vesicles cDNA, Rhesus

UD-407 30 reactions
EUR 316

cDNA - Monkey (Rhesus) Normal Tissue: Lung

C1534152 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Skin

C1534218 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Brain

C1534035 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Cecum

C1534089 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Colon

C1534090 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Heart

C1534122 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Liver

C1534149 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Ovary

C1534183 40 reactions
EUR 1311

cDNA - Monkey (Rhesus) Normal Tissue: Penis

C1534194 40 reactions
EUR 723

Monkey Eye cDNA-Random Primer, Rhesus

UD-106-RH 30 reactions
EUR 316

cDNA - Monkey (Rhesus) Normal Tissue: Kidney

C1534142 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Rectum

C1534206 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Spleen

C1534246 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Testis

C1534260 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Throat

C1534263 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Thymus

C1534264 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Tongue

C1534267 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Tonsil

C1534268 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Ureter

C1534273 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Uterus

C1534274 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Vagina

C1534283 40 reactions
EUR 723

Monkey Skin cDNA-Random Primer, Rhesus

UD-101-RH 30 reactions
EUR 316

Monkey Lung cDNA-Random Primer, Rhesus

UD-601-RH 30 reactions
EUR 316

cDNA - Monkey (Rhesus) Normal Tissue: Adipose

C1534003 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Adrenal

C1534004 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Bladder

C1534010 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Trachea

C1534160 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Parotid

C1534190 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Stomach

C1534248 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Thyroid

C1534265 40 reactions
EUR 723

Monkey Brain, cDNA-Random Primer, Rhesus

UD-201-RH 30 reactions
EUR 316

Monkey Small Intestine, Ileum cDNA, Rhesus

UD-309 30 reactions
EUR 316

Monkey Cecum cDNA-Random Primer, Rhesus

UD-310-RH 30 reactions
EUR 316

Monkey Colon cDNA-Random Primer, Rhesus

UD-311-RH 30 reactions
EUR 316

Monkey Liver cDNA-Random Primer, Rhesus

UD-314-RH 30 reactions
EUR 316

Monkey Heart cDNA-Random Primer, Rhesus

UD-801-RH 30 reactions
EUR 316

Monkey Aorta cDNA-Random Primer, Rhesus

UD-807-RH 30 reactions
EUR 446

cDNA - Monkey (Rhesus) Normal Tissue: Appendix

C1534006 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Pancreas

C1534188 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Placenta

C1534200 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Prostate

C1534201 40 reactions
EUR 723

Monkey Tongue cDNA-Random Primer, Rhesus

UD-105-RH 30 reactions
EUR 316

Monkey Rectum cDNA-Random Primer, Rhesus

UD-312-RH 30 reactions
EUR 316

Monkey Spleen cDNA-Random Primer, Rhesus

UD-701-RH 30 reactions
EUR 316

Monkey Thymus cDNA-Random Primer, Rhesus

UD-702-RH 30 reactions
EUR 316

Monkey Tonsil cDNA-Random Primer, Rhesus

UD-707-RH 30 reactions
EUR 446

Monkey Artery cDNA-Random Primer, Rhesus

UD-810-RH 30 reactions
EUR 446

Monkey Kidney cDNA-Random Primer, Rhesus

UD-901-RH 30 reactions
EUR 316

cDNA - Monkey (Rhesus) Normal Tissue: Esophagus

C1534106 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Diaphragm

C1534169 40 reactions
EUR 723

Monkey Stomach cDNA-Random Primer, Rhesus

UD-302-RH 30 reactions
EUR 316

Monkey Small Intestine, Jejunum cDNA, Rhesus

UD-308 30 reactions
EUR 316

Monkey Adrenal cDNA-Random Primer, Rhesus

UD-501-RH 30 reactions
EUR 446

Monkey Thyroid cDNA-Random Primer, Rhesus

UD-503-RH 30 reactions
EUR 446

Monkey Trachea cDNA-Random Primer, Rhesus

UD-602-RH 30 reactions
EUR 446

Monkey Bladder cDNA-Random Primer, Rhesus

UD-902-RH 30 reactions
EUR 446

cDNA - Monkey (Rhesus) Normal Tissue: Epididymis

C1534105 40 reactions
EUR 723

Monkey Small Intestine, Duodenum cDNA, Rhesus

UD-307 30 reactions
EUR 316

Monkey Prostate cDNA-Random Primer, Rhesus

UD-408-RH 30 reactions
EUR 316

cDNA - Monkey (Rhesus) Normal Tissue: Gallbladder

C1534118 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Parathyroid

C1534189 40 reactions
EUR 1475

Monkey Esophagus cDNA-Random Primer, Rhesus

UD-301-RH 30 reactions
EUR 316

Monkey Diaphragm cDNA-Random Primer, Rhesus

UD-605-RH 30 reactions
EUR 316

cDNA - Monkey (Rhesus) Normal Tissue: Lymph Node

C1534161 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Spinal Cord

C1534234 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Colon Sigmoid

C1534095 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Cervix

C1534275 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Corpus

C1534276 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Fundus

C1534278 40 reactions
EUR 723

Monkey Spinal cord cDNA-Random Primer, Rhesus

UD-230-RH 30 reactions
EUR 316

Monkey Lymph Nodes cDNA-Random Primer, Rhesus

UD-703-RH 30 reactions
EUR 446

cDNA - Monkey (Rhesus) Normal Tissue: Fallopian Tube

C1534115 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Stomach: Cardia

C1534250 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Stomach: Corpus

C1534251 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Stomach: Fundus

C1534252 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Colon Ascending

C1534091 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Ductus Deferens

C1534100 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Skeletal Muscle

C1534171 40 reactions
EUR 481

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Stomach: Pylorus

C1534253 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Colon descending

C1534092 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Colon Transverse

C1534096 40 reactions
EUR 723

Monkey Adipose Tissue cDNA-Random Primer, Rhesus

UD-103-RH 30 reactions
EUR 446

Monkey Salivary gland, cDNA-Random Primer, Rhesus

UD-317-RH 30 reactions
EUR 316

Monkey Tissue cDNA Panel, Rhesus, any 10 tissues

UD-010 10x10 reactions
EUR 1529

Cathepsin K (CTSK) Monoclonal Antibody (Rhesus monkey)

4-MAA267Si21
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK)

cDNA - Monkey (Rhesus) Normal Tissue: Heart: Pericardium

C1534133 40 reactions
EUR 723

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey)

4-MAA080Si21
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8)

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey)

4-MAA080Si22
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8)

Monkey Skeletal Muscles cDNA-Random Primer, Rhesus

UD-102-RH 30 reactions
EUR 316

Interleukin 5 (IL5) Polyclonal Antibody (Rhesus monkey)

4-PAA078Si01
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5)

Interleukin 6 (IL6) Polyclonal Antibody (Rhesus monkey)

4-PAA079Si01
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6)

Interleukin 8 (IL8) Polyclonal Antibody (Rhesus monkey)

4-PAA080Si01
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8)

cDNA - Monkey (Rhesus) Normal Tissue: Liver: Left Lobe

C1534150 40 reactions
EUR 481

Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey)

4-PAA979Si01
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA)

cDNA - Monkey (Rhesus) Normal Tissue: Liver: Right Lobe

C1534151 40 reactions
EUR 481

Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey)

4-PAA033Si01
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa)

Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey)

4-PAA546Si01
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA)

cDNA - Monkey (Rhesus) Normal Tissue: Blood Vessel: Vein

C1534020 40 reactions
EUR 723

Cathepsin K (CTSK) Monoclonal Antibody (Rhesus monkey), PE

4-MAA267Si21-PE
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with PE.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), PE

4-MAA080Si21-PE
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with PE.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), PE

4-MAA080Si22-PE
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with PE.

Cathepsin K (CTSK) Monoclonal Antibody (Rhesus monkey), APC

4-MAA267Si21-APC
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with APC.

Cathepsin K (CTSK) Monoclonal Antibody (Rhesus monkey), Cy3

4-MAA267Si21-Cy3
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with Cy3.

Cathepsin K (CTSK) Monoclonal Antibody (Rhesus monkey), HRP

4-MAA267Si21-HRP
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with HRP.

cDNA - Monkey (Rhesus) Normal Tissue: Blood Vessel: Artery

C1534013 40 reactions
EUR 723

Interleukin 5 (IL5) Polyclonal Antibody (Rhesus monkey), PE

4-PAA078Si01-PE
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with PE.

Interleukin 6 (IL6) Polyclonal Antibody (Rhesus monkey), PE

4-PAA079Si01-PE
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with PE.

Interleukin 8 (IL8) Polyclonal Antibody (Rhesus monkey), PE

4-PAA080Si01-PE
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with PE.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), APC

4-MAA080Si21-APC
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with APC.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), Cy3

4-MAA080Si21-Cy3
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Cy3.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), HRP

4-MAA080Si21-HRP
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with HRP.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), APC

4-MAA080Si22-APC
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with APC.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), Cy3

4-MAA080Si22-Cy3
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Cy3.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), HRP

4-MAA080Si22-HRP
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with HRP.

Cathepsin K (CTSK) Monoclonal Antibody (Rhesus monkey), FITC

4-MAA267Si21-FITC
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with FITC.

Interleukin 5 (IL5) Polyclonal Antibody (Rhesus monkey), APC

4-PAA078Si01-APC
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with APC.

Interleukin 5 (IL5) Polyclonal Antibody (Rhesus monkey), Cy3

4-PAA078Si01-Cy3
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with Cy3.

Interleukin 5 (IL5) Polyclonal Antibody (Rhesus monkey), HRP

4-PAA078Si01-HRP
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with HRP.

Interleukin 6 (IL6) Polyclonal Antibody (Rhesus monkey), APC

4-PAA079Si01-APC
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with APC.

Interleukin 6 (IL6) Polyclonal Antibody (Rhesus monkey), Cy3

4-PAA079Si01-Cy3
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with Cy3.

Interleukin 6 (IL6) Polyclonal Antibody (Rhesus monkey), HRP

4-PAA079Si01-HRP
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with HRP.

Interleukin 8 (IL8) Polyclonal Antibody (Rhesus monkey), APC

4-PAA080Si01-APC
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with APC.

Interleukin 8 (IL8) Polyclonal Antibody (Rhesus monkey), Cy3

4-PAA080Si01-Cy3
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with Cy3.

Interleukin 8 (IL8) Polyclonal Antibody (Rhesus monkey), HRP

4-PAA080Si01-HRP
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with HRP.

Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), PE

4-PAA979Si01-PE
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with PE.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), FITC

4-MAA080Si21-FITC
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with FITC.

Interleukin 8 (IL8) Monoclonal Antibody (Rhesus monkey), FITC

4-MAA080Si22-FITC
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Description: A Mouse monoclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with FITC.

Interleukin 6 (IL6) Monoclonal Antibody (Human, Rhesus monkey)

4-MAA079Si22
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  • 100ul
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Description: A Mouse monoclonal antibody against Human, Rhesus monkey Interleukin 6 (IL6)

Hemoglobin (HB) Polyclonal Antibody (Human, Rhesus monkey, Dog)

4-PAB409Si01
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human, Rhesus monkey, Dog Hemoglobin (HB)

Interleukin 5 (IL5) Polyclonal Antibody (Rhesus monkey), FITC

4-PAA078Si01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 5 (IL5). This antibody is labeled with FITC.

Interleukin 6 (IL6) Polyclonal Antibody (Rhesus monkey), FITC

4-PAA079Si01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with FITC.

Interleukin 8 (IL8) Polyclonal Antibody (Rhesus monkey), FITC

4-PAA080Si01-FITC
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interleukin 8 (IL8). This antibody is labeled with FITC.

Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), APC

4-PAA979Si01-APC
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with APC.

Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), Cy3

4-PAA979Si01-Cy3
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with Cy3.

Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), HRP

4-PAA979Si01-HRP
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with HRP.

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

C1534227 40 reactions
EUR 723

Carbonic Anhydrase II (CA2) Monoclonal Antibody (Rhesus monkey)

4-MAA782Si22
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  • 100ul
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Description: A Mouse monoclonal antibody against Rhesus monkey Carbonic Anhydrase II (CA2)

Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), PE

4-PAA033Si01-PE
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with PE.

Cyclophilin A (CYPA) Polyclonal Antibody (Rhesus monkey), FITC

4-PAA979Si01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Cyclophilin A (CYPA). This antibody is labeled with FITC.

Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), PE

4-PAA546Si01-PE
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with PE.

Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), APC

4-PAA033Si01-APC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with APC.

Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), Cy3

4-PAA033Si01-Cy3
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with Cy3.

Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), HRP

4-PAA033Si01-HRP
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with HRP.

Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), APC

4-PAA546Si01-APC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with APC.

Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), Cy3

4-PAA546Si01-Cy3
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with Cy3.

Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), HRP

4-PAA546Si01-HRP
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with HRP.

Interferon Alpha (IFNa) Polyclonal Antibody (Rhesus monkey), FITC

4-PAA033Si01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Interferon Alpha (IFNa). This antibody is labeled with FITC.

Immunoglobulin A (IgA) Polyclonal Antibody (Rhesus monkey), FITC

4-PAA546Si01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Rhesus monkey Immunoglobulin A (IgA). This antibody is labeled with FITC.

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 723

Monkey Small Intestine, Ileum cDNA-Random Primer, Rhesus

UD-309-RH 30 reactions
EUR 316

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 723

Cathepsin K (CTSK) Monoclonal Antibody (Rhesus monkey), Biotinylated

4-MAA267Si21-Biotin
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Description: A Mouse monoclonal antibody against Rhesus monkey Cathepsin K (CTSK). This antibody is labeled with Biotin.

Glycated Hemoglobin A1c (HbA1c) Polyclonal Antibody (Rhesus monkey)

4-PAA190Si01
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Description: A Rabbit polyclonal antibody against Rhesus monkey Glycated Hemoglobin A1c (HbA1c)

Monkey Small Intestine, Jejunum cDNA-Random Primer, Rhesus

UD-308-RH 30 reactions
EUR 316

Interleukin 6 (IL6) Monoclonal Antibody (Human, Rhesus monkey), PE

4-MAA079Si22-PE
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  • 100ul
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Description: A Mouse monoclonal antibody against Human, Rhesus monkey Interleukin 6 (IL6). This antibody is labeled with PE.
 

Synthesis of Full-Measurement cDNA Infectious Clones of Soybean Mosaic Virus and Purposeful Identification of a Key Amino Acid inside the Silencing Suppressor Hc-Skilled 

  • Soybean mosaic virus (SMV), which belongs to the Potyviridae, causes important reductions in soybean yield and seed top quality. On this look at, every tag-free and reporter gene inexperienced fluorescent protein (GFP)-containing infectious clones for the SMV N1 strain have been constructed by Gibson assembly and with the yeast homologous recombination system, respectively.
  • Every infectious clones are acceptable for agroinfiltration on the model host benthamiana and current sturdy infectivity for the pure host soybean and several other different totally different legume species. Every infectious clones have been seed transmitted and prompted typical virus indicators on seeds and progeny crops. We used the SMV-GFP infectious clone to extra look at the perform of key amino acids inside the silencing suppressor helper component-proteinase (Hc-Skilled).
  • Amongst twelve amino acid substitution mutants, the co-expression of mutant 2-with an Asparagine→Leucine substitution at place 182 of the FRNK (Phe-Arg-Asn-Lys) motif-attenuated viral indicators and alleviated the host progress retardation introduced on by SMV.
  • Moreover, the Hc-Prom2 mutant confirmed stronger oligomerization than wild-type Hc-Skilled. Taken collectively, the SMV infectious clones may be useful for analysis of host-SMV interactions and helpful gene characterization in soybeans and related legume species, notably by means of seed transmission properties. Furthermore, the SMV-GFP infectious clone will even facilitate helpful analysis of every virus and host genes in an benthamiana transient expression system. 

Profiling of rice Cd-tolerant genes by means of yeast-based cDNA library survival screening

The bioaccumulation of cadmium (Cd) in crop and the following meals chain has aroused intensive concerns. However, the underlying molecular mechanisms of plant Cd tolerance keep to be clarified from the viewpoint of novel candidate genes.

 

Proper right here we described a extraordinarily setting pleasant technique for preliminary determining rice Cd-tolerant genes by means of the yeast-based cDNA library survival screening blended with high-throughput sequencing approach. About 690 gene isoforms have been acknowledged as being Cd-tolerant candidates using this shotgun technique.

Among the many many Cd-tolerant genes acknowledged, numerous courses of genes paying homage to BAX inhibitor (BI), NAC transcription parts and Quick ALkalinization Parts (RALFs) have been of particular curiosity, and their carry out of Cd tolerance was extra validated by way of heterologous expression, which urged that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves.

Referring to the genes involved in ion transport, the validated Cd-tolerant heavy metal-associated space (HMAD) isoprenylated protein HIPP42 was considerably noteworthy. Further elucidation of these genes associated to Cd tolerance in rice will revenue agricultural actions.

Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Period for scRNA-Seq

 

Single-cell RNA-sequencing (scRNA-seq) permits a whole analysis of the transcriptome of specific particular person cells by next-generation sequencing. ScRNA-seq offers an unbiased technique to research the cell heterogeneity and dynamics of varied natural strategies, along with the immune system. Optimization of the technical procedures carried out earlier to RNA-seq analysis is essential to the success of a scRNA-seq experiment.

Proper right here, three major experimental procedures are described: (1) the isolation of immune CD8a+ T cells from major murine tissue, (2) the expertise of single-cell cDNA libraries using the 10× Genomics Chromium Controller and the Chromium Single Cell 3′ Reply, and (3) cDNA library top quality administration. On this protocol, CD8a+ T cells are isolated from murine spleen tissue, nonetheless any cell form of curiosity might be enriched and used for single-cell cDNA library expertise and subsequent RNA-seq experiments.

Combinatorial dynamics of protein synthesis time delay and detrimental suggestions loop in NF- κ B signalling pathway

The transcription issue NF-κB hyperlinks immune response and inflammatory response and its totally different oscillation patterns decide totally different cell fates. On this research, a mathematical mannequin with IκBα protein synthesis time delay is developed primarily based on the experimental evidences.

The outcomes present that point delay has the flexibility to drive oscillation of NF-κB through Hopf bifurcation. In the meantime, the amplitude and interval are delicate to the time delay. Furthermore, the time delay threshold is a perform of 4 parameters characterising the detrimental suggestions loop.

Likewise, the parameters additionally affect the amplitude and interval of NF-κB oscillation induced by time delay. Subsequently, the oscillation patterns of NF-κB are collaborative outcomes of time delay coupled with the detrimental suggestions loop. These outcomes not solely improve the understanding of NF-κB organic oscillation but in addition present clues for the event of anti-inflammatory or anti-cancer medicine.

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