replication by a cDNA expression cloning mixed with MinION sequencing

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jdomenech

Identification of cellular inhibitors in opposition to Chikungunya virus replication by a cDNA expression cloning combined with MinION sequencing

  • cDNA expression cloning has been confirmed to be a robust technique inside the look for cellular parts that administration virus replication. On this look at, cDNA library screening using a pool of cDNA derived from interferon-treated human cells was combined with the MinION sequencer to ascertain cellular genes inhibiting Chikungunya virus (CHIKV) replication.
  • Drawback an an infection of CHIKV to Vero cells transduced with the cDNA library produced virus-resistant cells. Then, the MinION sequence of cDNAs extracted from the surviving cells revealed that the open finding out frames of TOM7, S100A16, N-terminally truncated kind of ECI1 (ECI1ΔN59), and RPL29 have been inserted in plenty of the cells.
  • Importantly, the transient expression of TOM7, S100A16, and ECI1ΔN59 was found to inhibit the replication of CHIKV in Huh7 cells, indicating that these cellular parts have been doubtlessly anti-CHIKV molecules.
  • Thus, our look at demonstrated that cDNA expression cloning combined with the MinION sequencer allowed a quick and full detection of cellular inhibitors in opposition to CHIKV.

    jdomenech
    jdomenech

XPO6

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Lenti ORF clone of Human exportin 6 (XPO6), mGFP tagged

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3`UTR clone of exportin 6 (XPO6) for miRNA target validation

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Lenti ORF clone of Human exportin 6 (XPO6), Myc-DDK-tagged

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20-abx940017
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  • 15 nmol
  • 30 nmol

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  • 15 nmol
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XPO6 Antibody

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  • 150ul
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Description: A polyclonal antibody against XPO6. Recognizes XPO6 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC

XPO6 Antibody

E046712 100μg/100μl
EUR 255
Description: Available in various conjugation types.

XPO6 Antibody

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EUR 420

XPO6 Antibody

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EUR 280

XPO6 Antibody

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EUR 350

XPO6 Antibody

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EUR 275
Description: Available in various conjugation types.

XPO6 Antibody

46712 100ul
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XPO6 Antibody

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EUR 289
Description: Rabbit polyclonal XPO6 antibody

XPO6 Antibody

1-CSB-PA842747ESR1HU
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  • 100ul
  • 50ul
Description: A polyclonal antibody against XPO6. Recognizes XPO6 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Xpo6 (Myc-DDK-tagged) - Mouse exportin 6 (Xpo6)

MR211663 10 µg Ask for price

XPO6 (Myc-DDK-tagged)-Human exportin 6 (XPO6)

RC214839 10 µg Ask for price

Xpo6 (untagged ORF) - Rat exportin 6 (Xpo6), (10 ug)

RN204356 10 µg Ask for price

XPO6 rabbit pAb

E28PT7008 100μl
EUR 255
Description: Available in various conjugation types.

XPO6 Rabbit pAb

A10401-100ul 100 ul
EUR 369.6

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A10401-200ul 200 ul
EUR 550.8

XPO6 Rabbit pAb

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EUR 219.6

XPO6 Rabbit pAb

A10401-50ul 50 ul
EUR 267.6

XPO6 rabbit pAb

E44H15561 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Mouse XPO6 siRNA

abx940017-150g 150 µg
EUR 450

Mouse XPO6 siRNA

abx940017-300g 300 µg
EUR 612.5

Human XPO6 siRNA

abx940018-150g 150 µg
EUR 450

Human XPO6 siRNA

abx940018-300g 300 µg
EUR 612.5

anti- XPO6 antibody

FNab09550 100µg
EUR 606.3
Description: Antibody raised against XPO6

XPO6 Blocking Peptide

DF12799-BP 1mg
EUR 234

XPO6 Polyclonal Antibody

E910401 100ul
EUR 225
Description: Available in various conjugation types.

XPO6 Conjugated Antibody

C46712 100ul
EUR 476.4

XPO6 (untagged) - Homo sapiens exportin 6 (XPO6), transcript variant 1

SC333010 10 µg Ask for price

Xpo6 (Myc-DDK-tagged ORF) - Rat exportin 6 (Xpo6), (10 ug)

RR204356 10 µg Ask for price

XPO6 ELISA KIT|Human

EF004323 96 Tests
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XPO6 (GFP-tagged) - Homo sapiens exportin 6 (XPO6), transcript variant 1

RG235170 10 µg Ask for price

Human XPO6 shRNA Plasmid

20-abx958092
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  • 150 µg
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Mouse XPO6 shRNA Plasmid

20-abx978151
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  • 150 µg
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Exportin 6 (XPO6) Antibody

20-abx112420
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  • 150 ul
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Exportin 6 (XPO6) Antibody

abx112420-100l 100 µl
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Exportin-6 (XPO6) Antibody

20-abx321354
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Exportin-6 (XPO6) Antibody

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Exportin-6 (XPO6) Antibody

20-abx126798
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Exportin-6 (XPO6) Antibody

abx239550-100ug 100 ug
EUR 577.2

Exportin-6 (XPO6) Antibody

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EUR 337.5

Exportin-6 (XPO6) Antibody

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Exportin-6 (XPO6) Antibody

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Recombinant Exportin 6 (XPO6)

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EUR 431.2

Recombinant Exportin 6 (XPO6)

RPU50738-1mg 1mg
EUR 1911

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OACA09320-50UL - XPO6 Antibody

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OACA09320-100UL - XPO6 Antibody

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XPO6 Rabbit Polyclonal Antibody

E10G09269 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

XPO6 Rabbit Polyclonal Antibody

E10G02298 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

XPO6 (Myc-DDK tagged) - Homo sapiens exportin 6 (XPO6), transcript variant 1

RC235170 10 µg Ask for price

Xpo6 ORF Vector (Rat) (pORF)

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Xpo6 ORF Vector (Mouse) (pORF)

ORF061968 1.0 ug DNA
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XPO6 ORF Vector (Human) (pORF)

ORF014966 1.0 ug DNA
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XPO6 ORF Vector (Human) (pORF)

ORF011649 1.0 ug DNA
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Human XPO6 knockout cell line

ABC-KH17093 1 vial Ask for price
Description: Human XPO6 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human Exportin 6 (XPO6) Protein

20-abx650936
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  • 100 ug
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Human XPO6 knockdown cell line

ABC-KD17093 1 vial Ask for price
Description: Human XPO6 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human XPO6 Protein Lysate 20ug

IHUXPO6PLLY20UG each
EUR 361
Description: Human XPO6 Protein Lysate 20ug

Human Exportin 6 (XPO6) Protein

abx650936-5mg 5 mg
EUR 337.5

Lenti ORF particles, Xpo6 (GFP-tagged) - Mouse exportin 6 (Xpo6), 200ul, >10^7 TU/mL

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Lenti ORF particles, XPO6 (mGFP-tagged) - Human exportin 6 (XPO6), 200ul, >10^7 TU/mL

RC214839L4V 200 µl Ask for price

OCOA10796-20UG - XPO6 Protein Lysate

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Lenti ORF particles, Xpo6 (GFP-tagged ORF) - Rat exportin 6 (Xpo6), 200ul, >10^7 TU/mL

RR204356L4V 200 µl Ask for price

Lenti ORF particles, XPO6 (Myc-DDK tagged) - Human exportin 6 (XPO6), 200ul, >10^7 TU/mL

RC214839L3V 200 µl Ask for price

Lenti ORF particles, Xpo6 (Myc-DDK-tagged) - Mouse exportin 6 (Xpo6), 200ul, >10^7 TU/mL

MR211663L3V 200 µl Ask for price

Mouse Exportin- 6, Xpo6 ELISA KIT

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Human Exportin- 6, XPO6 ELISA KIT

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Human Exportin 6(XPO6)ELISA Kit

QY-E01489 96T
EUR 433.2

Human Exportin-6 (XPO6) ELISA Kit

abx384332-96tests 96 tests
EUR 1093.2

Mouse Exportin-6 (XPO6) ELISA Kit

abx553736-96tests 96 tests
EUR 687.5

Lenti ORF particles, Xpo6 (Myc-DDK-tagged ORF) - Rat exportin 6 (Xpo6), 200ul, >10^7 TU/mL

RR204356L3V 200 µl Ask for price

Xpo6 sgRNA CRISPR Lentivector set (Rat)

K6323501 3 x 1.0 ug
EUR 406.8

Lenti ORF clone of 1110017D15Rik (mGFP-tagged) - Mouse RIKEN cDNA 1110017D15 gene (cDNA clone IMAGE:6741413)

MR200011L4 10 µg Ask for price

Xpo6 sgRNA CRISPR Lentivector set (Mouse)

K4963501 3 x 1.0 ug
EUR 406.8

XPO6 sgRNA CRISPR Lentivector set (Human)

K2650901 3 x 1.0 ug
EUR 406.8

XPO6 3'UTR GFP Stable Cell Line

TU078606 1.0 ml
EUR 1672.8

Xpo6 3'UTR GFP Stable Cell Line

TU172394 1.0 ml Ask for price

Xpo6 3'UTR GFP Stable Cell Line

TU273481 1.0 ml Ask for price

Lenti ORF clone of 1110017D15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110017D15 gene (cDNA clone IMAGE:6741413)

MR200011L3 10 µg Ask for price

XPO6 Protein Vector (Rat) (pPM-C-HA)

PV316868 500 ng
EUR 1429.2

Identification of a novel KIR3DL3*064 allele by cDNA cloning and sequencing

Objective: To report on a novel KIR3DL3 allele acknowledged in a southern Han Chinese language language specific individual.

Methods: Peripheral blood sample was collected from a voluntary blood donor with inconclusive consequence by KIR3DL3 sequence-based typing (SBT). Complete mRNA was extracted and subjected to reverse transcription to accumulate KIR3DL3 cDNA, which was then amplified by PCR with a pair of KIR3DL3-specific primers. The product was subjected to cDNA cloning and sequencing.

Outcomes: cDNA cloning and sequencing have acknowledged a wide-type KIR3DL3*00802 allele and a novel KIR3DL3*064 allele. The latter differed from KIR3DL3*00601 by a missense variant at codon 374[c.1184 C>T (p.Thr374Ile)] in exon 9. The novel KIR3DL3 allele has been formally assigned by the KIR subcommittee of World Nicely being Group Nomenclature Committee for parts of HLA system.

Conclusion: cDNA cloning and sequencing is also used to inform aside inconclusive ends in KIR3DL3 SBT with a goal to ascertain novel KIR alleles. 

Apple Russet Ring and Apple Inexperienced Crinkle Diseases: Achievement of Koch’s Postulates by Virome Analysis, Amplification of Full-Measurement cDNA of Viral Genomes, in vitro Transcription of Infectious Viral RNAs, and Reproduction of Indicators on Fruits of Apple Bushes Inoculated With Viral RNAs

Apple russet ring and apple inexperienced crinkle are graft-transmitted diseases first reported better than 60 years previously, nonetheless at present, no affiliation between a particular virus (variant) and the sickness has been clearly demonstrated.

On this look at, we carried out the following assortment of experiments to ascertain the causal viruses (variants) of these apple diseases; (1) full analysis by next-generation sequencing of all viruses in each apple tree affected with russet ring or inexperienced crinkle sickness,

(2) amplification of full-length genomic cDNA of viruses using primers containing the T3 promoter and the in vitro transcription of infectious viral RNAs, (3) inoculation of viral RNA transcripts to every herbaceous and apple crops, (4) analysis of sequence variants of viruses present in contaminated crops, (5) back-inoculation of sequence variants of candidate viruses to apple seedlings combined with the virus-induced flowering know-how using the apple latent spherical virus vector to breed the symptom on the fruit as shortly as doable, and

(6) duplicate of indicators on the fruits of apple timber inoculated with sequence variants and the re-isolation of each virus variant from apples exhibiting fruit indicators.

The outcomes confirmed that certainly one of many sequence variants of the apple chlorotic leaf spot virus causes a attribute ring-shaped rust on the fruits of contaminated apple timber and {{that a}} sequence variant of the apple stem pitting virus possibly causes inexperienced crinkle indicators on an contaminated apple fruit.

Thus, we’ve got been able to fulfill Koch’s postulates to point out the viral etiology of every the apple russet ring and inexperienced crinkle diseases. We moreover counsel an experimental system which will present whether or not or not a virus current in diseased tissues is the pathogen responsible for the diseases when the etiology is undetermined.

Artemisinin susceptibility within the malaria parasite Plasmodium falciparum: propellers, adaptor proteins and the necessity for mobile therapeutic

Research of the susceptibility of Plasmodium falciparum to the artemisinin household of antimalarial medication present a posh image of partial resistance (tolerance) related to elevated parasite survival in vitro and in vivo. We current an outline of the genetic loci that, in mutant kind, can independently elicit parasite tolerance.

These encode kelch propeller area protein PfK13, ubiquitin hydrolase UBP-1, actin filament-organising protein Coronin, additionally carrying a propeller area, and the trafficking adaptor subunit AP-2μ.

Detailed research of those proteins and the practical foundation of artemisinin tolerance in blood stage parasites are enabling a brand new synthesis of our understanding up to now. To information additional experimental work, we current two main conclusions.

Firstly, we suggest a dual-component mannequin of artemisinin tolerance in P. falciparum comprising suppression of artemisinin activation in early ring-stage by decreasing endocytic haemoglobin seize from host cytosol, coupled with enhancement of mobile therapeutic mechanisms in surviving cells.

Secondly, these two unbiased necessities restrict the chance of growth of full artemisinin resistance by P. falciparum, favouring deployment of current medication in new schedules designed to use these organic limits, thus extending the helpful lifetime of present mixture therapies.

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