replication by a cDNA expression cloning mixed with MinION sequencing

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jdomenech

Identification of cellular inhibitors in opposition to Chikungunya virus replication by a cDNA expression cloning combined with MinION sequencing

  • cDNA expression cloning has been confirmed to be a robust technique inside the look for cellular parts that administration virus replication. On this look at, cDNA library screening using a pool of cDNA derived from interferon-treated human cells was combined with the MinION sequencer to ascertain cellular genes inhibiting Chikungunya virus (CHIKV) replication.
  • Drawback an an infection of CHIKV to Vero cells transduced with the cDNA library produced virus-resistant cells. Then, the MinION sequence of cDNAs extracted from the surviving cells revealed that the open finding out frames of TOM7, S100A16, N-terminally truncated kind of ECI1 (ECI1ΔN59), and RPL29 have been inserted in plenty of the cells.
  • Importantly, the transient expression of TOM7, S100A16, and ECI1ΔN59 was found to inhibit the replication of CHIKV in Huh7 cells, indicating that these cellular parts have been doubtlessly anti-CHIKV molecules.
  • Thus, our look at demonstrated that cDNA expression cloning combined with the MinION sequencer allowed a quick and full detection of cellular inhibitors in opposition to CHIKV.

    jdomenech
    jdomenech

Genorise® Chicken IL-6 cDNA Clone

GR147003 10 µg
EUR 183.6

Genorise® Chicken IL-4 cDNA Clone

GR147004 10 µg
EUR 183.6

Genorise® Chicken IL-10 cDNA Clone

GR147005 10 µg
EUR 183.6

XPO6 Blocking Peptide

DF12799-BP 1mg
EUR 234

XPO6 Conjugated Antibody

C46712 100ul
EUR 476.4

XPO6 cloning plasmid

CSB-CL842747HU1-10ug 10ug
EUR 976.8
Description: A cloning plasmid for the XPO6 gene.

XPO6 cloning plasmid

CSB-CL842747HU2-10ug 10ug
EUR 1442.4
Description: A cloning plasmid for the XPO6 gene.

XPO6 Rabbit pAb

A10401-100ul 100 ul
EUR 369.6

XPO6 Rabbit pAb

A10401-200ul 200 ul
EUR 550.8

XPO6 Rabbit pAb

A10401-20ul 20 ul
EUR 219.6

XPO6 Rabbit pAb

A10401-50ul 50 ul
EUR 267.6

anti- XPO6 antibody

FNab09550 100µg
EUR 606.3
Description: Antibody raised against XPO6

Anti-XPO6 antibody

PAab09550 100 ug
EUR 426

Anti-XPO6 antibody

STJ112437 100 µl
EUR 332.4
Description: The protein encoded by this gene is a member of the importin-beta family. Members of this family are regulated by the GTPase Ran to mediate transport of cargo across the nuclear envelope. This protein has been shown to mediate nuclear export of profilin-actin complexes. A pseudogene of this gene is located on the long arm of chromosome 14. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

Anti-XPO6 antibody

STJ13100475 100 µl
EUR 512.4

Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11

MASF-5A-Clone-10E11 1 mg
EUR 667
Description:

Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5

MASF-5A-Clone-10G5 1 mg
EUR 667
Description:

Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Genorise® Human GLUT1 cDNA Clone, 10 ug

GR154002 10 µg
EUR 183.6

Genorise® Human cTnT cDNA Clone, 10 ug

GR154003 10 µg
EUR 183.6

Genorise® Human TNN3 cDNA Clone, 10 ug

GR154004 10 µg
EUR 183.6

Genorise® Human BNP cDNA Clone, 10 ug

GR154005 10 µg
EUR 183.6

Genorise® Human Myoglobin cDNA Clone, 10 ug

GR154006 10 µg
EUR 183.6

Genorise® Human ANP cDNA Clone, 10 ug

GR154008 10 µg
EUR 183.6

Genorise® Human Myeloperoxidase cDNA Clone, 10 ug

GR154009 10 µg
EUR 183.6

Genorise® Human GPBB cDNA Clone, 10 ug

GR154010 10 µg
EUR 183.6

Genorise® Human CRP cDNA Clone, 10 ug

GR154011 10 µg
EUR 183.6

Genorise® Human MyBPC3 cDNA Clone, 10 ug

GR154012 10 µg
EUR 183.6

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11

MALV-30A-Clone-3B11 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4

MALV-30A-Clone-5A4 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2

MALV-30A-Clone-6C2 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7

MALV-30A-Clone-6D7 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Human XPO6 shRNA Plasmid

20-abx958092
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse XPO6 shRNA Plasmid

20-abx978151
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Exportin-6 (XPO6) Antibody

20-abx126798
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Exportin 6 (XPO6) Antibody

20-abx112420
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Exportin-6 (XPO6) Antibody

abx037309-100ug 100 ug
EUR 469.2

Exportin-6 (XPO6) Antibody

20-abx321354
  • EUR 526.80
  • EUR 393.60
  • 100 ul
  • 50 ul

Exportin-6 (XPO6) Antibody

abx239550-100ug 100 ug
EUR 577.2

XPO6 ELISA KIT|Human

EF004323 96 Tests
EUR 826.8

Genorise® Sheep IL-6 cDNA Clone, 10 ug

GR151001 10 µg
EUR 183.6

Genorise® Ovine IL-2 cDNA Clone, 10 ug

GR151002 10 µg
EUR 183.6

Genorise® Ovine IL-4 cDNA Clone, 10 ug

GR151003 10 µg
EUR 183.6

Genorise® Bovine IL-1b cDNA Clone, 10 ug

GR152001 10 µg
EUR 183.6

Genorise® Rabbit IL-1b cDNA Clone, 10 ug

GR153001 10 µg
EUR 183.6

Genorise® Human H-FABP cDNA Clone, 10 ug

GR154001 10 µg
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Genorise® Human Lp-PLA2 cDNA Clone, 10 ug

GR154007 10 µg
EUR 183.6

Genorise® Human PAPP-A cDNA Clone, 10 ug

GR154013 10 µg
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Genorise® Human NT-proBNP cDNA Clone, 10 ug

GR154014 10 µg
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Genorise® Human Pro-C3 cDNA Clone, 10 ug

GR154015 10 µg
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Genorise® Canine NGF-B cDNA Clone, 10 ug

GR159001 10 µg
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Genorise® Canine IL-7 cDNA Clone, 10 ug

GR159002 10 µg
EUR 183.6

Human Exportin 6 (XPO6) Protein

20-abx650936
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Xpo6 ORF Vector (Rat) (pORF)

ORF079217 1.0 ug DNA
EUR 607.2

Xpo6 ORF Vector (Mouse) (pORF)

ORF061968 1.0 ug DNA
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XPO6 ORF Vector (Human) (pORF)

ORF014966 1.0 ug DNA
EUR 424.8

XPO6 ORF Vector (Human) (pORF)

ORF011649 1.0 ug DNA
EUR 114

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
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  • 100 rxns × 20 ul Systems
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Novo? cDNA Kit

M1165-100
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Novo? cDNA Kit

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Evo? cDNA Supermix

M1168-100
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Evo? cDNA Supermix

M1168-25
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Novo? cDNA Supermix

M1169-100
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Novo? cDNA Supermix

M1169-25
EUR 346.8

Human Exportin-6 (XPO6) ELISA Kit

abx384332-96tests 96 tests
EUR 1093.2

Mouse Exportin- 6, Xpo6 ELISA KIT

ELI-51533m 96 Tests
EUR 1038

XPO6 sgRNA CRISPR Lentivector set (Human)

K2650901 3 x 1.0 ug
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Human Exportin- 6, XPO6 ELISA KIT

ELI-17963h 96 Tests
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Xpo6 sgRNA CRISPR Lentivector set (Mouse)

K4963501 3 x 1.0 ug
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Xpo6 sgRNA CRISPR Lentivector set (Rat)

K6323501 3 x 1.0 ug
EUR 406.8

Human Exportin 6(XPO6)ELISA Kit

QY-E01489 96T
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cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 744.00
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 1045.20
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  • 50 rxns × 20 ul Systems

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Identification of a novel KIR3DL3*064 allele by cDNA cloning and sequencing

Objective: To report on a novel KIR3DL3 allele acknowledged in a southern Han Chinese language language specific individual.

Methods: Peripheral blood sample was collected from a voluntary blood donor with inconclusive consequence by KIR3DL3 sequence-based typing (SBT). Complete mRNA was extracted and subjected to reverse transcription to accumulate KIR3DL3 cDNA, which was then amplified by PCR with a pair of KIR3DL3-specific primers. The product was subjected to cDNA cloning and sequencing.

Outcomes: cDNA cloning and sequencing have acknowledged a wide-type KIR3DL3*00802 allele and a novel KIR3DL3*064 allele. The latter differed from KIR3DL3*00601 by a missense variant at codon 374[c.1184 C>T (p.Thr374Ile)] in exon 9. The novel KIR3DL3 allele has been formally assigned by the KIR subcommittee of World Nicely being Group Nomenclature Committee for parts of HLA system.

Conclusion: cDNA cloning and sequencing is also used to inform aside inconclusive ends in KIR3DL3 SBT with a goal to ascertain novel KIR alleles. 

Apple Russet Ring and Apple Inexperienced Crinkle Diseases: Achievement of Koch’s Postulates by Virome Analysis, Amplification of Full-Measurement cDNA of Viral Genomes, in vitro Transcription of Infectious Viral RNAs, and Reproduction of Indicators on Fruits of Apple Bushes Inoculated With Viral RNAs

Apple russet ring and apple inexperienced crinkle are graft-transmitted diseases first reported better than 60 years previously, nonetheless at present, no affiliation between a particular virus (variant) and the sickness has been clearly demonstrated.

On this look at, we carried out the following assortment of experiments to ascertain the causal viruses (variants) of these apple diseases; (1) full analysis by next-generation sequencing of all viruses in each apple tree affected with russet ring or inexperienced crinkle sickness,

(2) amplification of full-length genomic cDNA of viruses using primers containing the T3 promoter and the in vitro transcription of infectious viral RNAs, (3) inoculation of viral RNA transcripts to every herbaceous and apple crops, (4) analysis of sequence variants of viruses present in contaminated crops, (5) back-inoculation of sequence variants of candidate viruses to apple seedlings combined with the virus-induced flowering know-how using the apple latent spherical virus vector to breed the symptom on the fruit as shortly as doable, and

(6) duplicate of indicators on the fruits of apple timber inoculated with sequence variants and the re-isolation of each virus variant from apples exhibiting fruit indicators.

The outcomes confirmed that certainly one of many sequence variants of the apple chlorotic leaf spot virus causes a attribute ring-shaped rust on the fruits of contaminated apple timber and {{that a}} sequence variant of the apple stem pitting virus possibly causes inexperienced crinkle indicators on an contaminated apple fruit.

Thus, we’ve got been able to fulfill Koch’s postulates to point out the viral etiology of every the apple russet ring and inexperienced crinkle diseases. We moreover counsel an experimental system which will present whether or not or not a virus current in diseased tissues is the pathogen responsible for the diseases when the etiology is undetermined.

Artemisinin susceptibility within the malaria parasite Plasmodium falciparum: propellers, adaptor proteins and the necessity for mobile therapeutic

Research of the susceptibility of Plasmodium falciparum to the artemisinin household of antimalarial medication present a posh image of partial resistance (tolerance) related to elevated parasite survival in vitro and in vivo. We current an outline of the genetic loci that, in mutant kind, can independently elicit parasite tolerance.

These encode kelch propeller area protein PfK13, ubiquitin hydrolase UBP-1, actin filament-organising protein Coronin, additionally carrying a propeller area, and the trafficking adaptor subunit AP-2μ.

Detailed research of those proteins and the practical foundation of artemisinin tolerance in blood stage parasites are enabling a brand new synthesis of our understanding up to now. To information additional experimental work, we current two main conclusions.

Firstly, we suggest a dual-component mannequin of artemisinin tolerance in P. falciparum comprising suppression of artemisinin activation in early ring-stage by decreasing endocytic haemoglobin seize from host cytosol, coupled with enhancement of mobile therapeutic mechanisms in surviving cells.

Secondly, these two unbiased necessities restrict the chance of growth of full artemisinin resistance by P. falciparum, favouring deployment of current medication in new schedules designed to use these organic limits, thus extending the helpful lifetime of present mixture therapies.

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